You'll be travelling north to south as you cover the route. Start from the Church of San Francesco in Gubbio, located on the southern end of Piazza Quaranta Martiri at the foot of the old city. The frescoes done by Ottaviano Nelli depicting stories of the Virgin were done in the style of international Gothic.

Perugia under the Baglioni family was undergoing innovative artistic change and even Domenico Veneziano painted for the ruling noble family. These frescoes have been lost to us, but you can still see the great works done by the artist in the Domenico di Bartolo polyptych and the Triptych by Beato Angelico, both housed at the Galleria Nazionale dell'Umbria in the city centre.

As you continue southwards along the valley, stop in to see Palazzo Trinci, located at the northern end of Piazza della Repubblica in Foligno. The Trinci family commissioned the decoration of their palazzo to various artists and Gentile da Fabriano seems to have been the one to paint the Sala dei Giganti with large effigies of the illustrious men of Ancient Rome. The palazzo is also home to the city museum.