This route, like route 2, starts from Umbertide’s Piazza Mazzini, and features two noticeable climbs connecting the Upper Tiber Valley with Lake Trasimeno. Most of the roads taken are very quiet, with very little traffic at anytime, and the spectacular views are more than just reward for the energy spent. It is not a particularly long route, but the two long climbs (Colle Campana and Valico Gosparini), although not steep, require a considerable level of fitness.

The first uphill section is encountered a mere 600 metres out of Umbertide, going towards Città di Castello, where you turn left towards Castel Rigone and Preggio: this marks the beginning of the first long climb of the day – roughly 15 kilometres – up to Colle Campana at 750 metres above sea level. This climb is not particularly steep (and there are a couple of kilometres of descent in between) with the exception of the very last section leading up to the top of the hill. There is a splendid view when you make it to the top: to your left, Monte Tezio and the Umbria-Marche Apennines, and to your right, Lake Trasimeno and beyond as far as Monte Amiata in Tuscany. The road continues rises and falls for several kilometres until you get to the village of Castel Rigone, situated at km. 18. You can stop here for a rest and a bite to eat, before beginning the highly panoramic descent down to the shores of Lake Trasimen.

At the end of the descent (km. 27) turn right towards Passignano, and then continue along the SS75bis as far as Tuoro, before turning right and following the signs for Umbertide and Lisciano Niccone. The road out of Tuoro marks the beginning of the 6-kilometre climb up to Valico Gosparini, where you should stop a while to enjoy the marvellous view of Lake Trasimeno below. You now descend towards Lisciano Niccone, and at the end of the descent (km. 49) bear left in the direction of Umbertide. The following section of the ride takes in the entire Niccone Valley, characterised by its geometrical plots of farmland, each with different crops.

After a total of 51 kilometres you come to Mengaccini, where you can fill your water bottle at a fountain on the right, but should you miss this you can do likewise at the village of Niccone at km. 66.5. When you reach the junction with the SS3bis at Niccone, turn right and ride back to the starting point in Umbertide, another 4 kilometres further on. However, should you feel like extending your ride, you can turn left at the same junction, in the direction of Città di Castello. About one kilometre past the village of Montecastelli, turn right off the SS3bis onto the local road that climbs up for about 4 km. to the beautiful medieval town of Montone. After a brief visit to this unique little town, classified among the most beautiful small towns in Italy, follow the signs to Umbertide, which is another 10 km. further on, most of which being downhill.

Apart from Umbertide town centre, which is described in route 2, this particular ride is characterised by the beautiful natural landscape, which varies from the rather mountainous Colle Campana and Castel Rigone, to the lakeside habitat of Trasimeno. Castel Rigone has its Sanctuario di Maria Santissima dei Miracoli, built towards the end of the 15th century by a pupil of the famous architect Bramante, and generally considered to be one of the finest examples of Renaissance architecture in Umbria. The lakeside town of Tuoro was the site of the historical "Battle of Trasimeno" in 217 BC between the Romans and the Carthaginians: a special itinerary has been laid out for visitors, retracing the main phases of the famous defeat of the Roman army at the hands of Hannibal’s troops.